Press release
Transcreener® GDP GTPase Assay - The Only Mix-and-Read Fluorescent GTPase Assay, by BellBrook Labs
GDP-Assay-Schematic
BellBrook Labs' Transcreener GDP GTPase assays are available with fluorescence polarization (FP), fluorescence intensity (FI), and far-red, time-resolved Forster-resonance-energy-transfer (TR-FRET) readouts that provide a safe, HTS-compatible alternative to cumbersome radioassay methods and are more sensitive and less subject to interference than colorimetric phosphate detection methods.
Based on BellBrook Labs' proprietary nucleotide immunodetection technology, the Transcreener® GDP GTPase Assays are compatible with any enzyme class that produces GDP, including monomeric small G proteins, such as CDC42, and Gα subunits of heterotrimeric G proteins, such as Gαi1.
GDP displaces tracer from a highly selective monoclonal antibody resulting in a decrease in fluorescence polarization or an increase in fluorescence intensity.
• Measure guanine nucleotide exchange factor (GEF) activity
• Measure GTPase-activating proteins (GAP) activity
• Homogenous, simple, mix-and-read assay
• Tunable dynamic range to match the target of interest
• Detect any GDP producing enzyme: GTPases, fucosyltransferases, mannosyltransferases
Three Readouts Available
• Fluorescence polarization (FP)
• Fluorescence intensity (FI)
• Time-resolved Forster-resonance-energy-transfer (TR-FRET)
Assay Features
• Easy-to-Use, Ultra-Sensitive GTPase Activity Assay
• HTS Compatible with 96, 384, and 1536-well plates
• Direct detection of GDP to monitor GTPase, GAP, and GEF Activity
• Stable assay reagents – minimum 8-hour reagent and signal stability
Far Red FP, FI &TR-FRET Readouts Validated on Major Multimode Readers
Model
Tecan
Infinite® M200, Infinite® F200/Ultra evolution, Infinite® F500, Infinite® M1000/M1000Pro/ Safire2™, Spark™ 10M
PerkinElmer
EnVision®/EnVision® Xcite
Molecular Devices
Analyst® GT/ HT, Gemini® XPS/EM, Spectra-Max® M2/M2e, SpectraMax® M5/M5e/ FlexStation® 3, SpectraMax® Paradigm
BMG Labtech
CLARIOstar®, FLUOstar® Omega, PHERAstar® FSX, PHERAstar® Plus/FS, POLARstar® Omega
BioTek
Cytation™ 5/3/1, Synergy™ H1, Synergy™ 2/H4 /4, Synergy™ HTX, Synergy™ Neo 2
Berthold
TriStar²S LB 942, Mithras² LB 943
All BellBrook Labs products can be ordered in Europe from MoBiTec: http://www.mobitec.com
Contact info@mobitec.com for more information and bulk pricing options.
About Bellbrook Labs
BellBrook Labs is dedicated to providing the pharmaceutical industry with enabling screening tools to accelerate the discovery of more effective therapies. As a small, R&D-driven company, BBL identifies areas where there is a strong need but a significant technical hurdle, and then BBL develops a solution. The company is focused on taking creative approaches to new product development and to continually improving the level of service provided to our customers. The company has built its reputation on Transcreener®, a biochemical assay platform. www.bellbrooklabs.com
About MoBiTec GmbH
MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.
In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.
MoBiTec GmbH, Lotzestr. 22a, 37083 Goettingen,
Germany, info@mobitec.com, www.mobitec.com
This release was published on openPR.
Permanent link to this press release:
Copy
Please set a link in the press area of your homepage to this press release on openPR. openPR disclaims liability for any content contained in this release.
You can edit or delete your press release Transcreener® GDP GTPase Assay - The Only Mix-and-Read Fluorescent GTPase Assay, by BellBrook Labs here
News-ID: 1241307 • Views: …
More Releases from MoBiTec GmbH
FerroFarRed - An Ion Indicator for the Specific Detection of Fe2+
Intracellular Fe2+ can react with hydrogen peroxide, thereby producing the hydroxyl radical, a reactive oxygen species (ROS). Excessive ROS causes oxidative stress, resulting in cancer and neurodegenerative diseases.
FerroFarRed (also known as SiRhoNox-1 or ER-SiRhoNox) is a fluorescent probe,manufactured by Goryo Chemical, Japan, distributed in Europe by MoBiTec GmbH, Germany. FerroFarRed specifically detects labile iron (II) ions (Fe2+) and is known to react with Fe2+ only, separately from other metal…
TransIT-VirusGEN® Transfection Reagent - Ideal for Recombinant Adeno-associated …
TransIT-VirusGEN® Transfection Reagent by Mirus, USA, is designed to enhance delivery of packaging and transfer vectors to adherent and suspension HEK 293 cell types to increase recombinant adeno-associated virus (AAV) and lentivirus production.
Adeno-associated virus (AAV) is a nonenveloped, single stranded DNA virus from the Paroviridae family notable for its lack of pathogenicity, low immunogenicity and ability to infect both dividing and quiescent cells. Because AAV is replication-defective in the…
Elabscience's Vast Portfolio of Products for Life Science Research Distributed b …
MoBiTec's most recent select partner Elabscience specializes in immunodiagnostic technology for the life science community, offering a complete platform for R&D and manufacture.
Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling Kits，Biochemical Kits, Cell Lines, and Other Reagents.
Elabscience also offers custom services for customers, including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service, and Whole Research Project Solutions.
Every…
MoBiTec to Exhibit at Potsdam Days on Bioanalysis 2018, Taking Place in Potsdam, …
The "Potsdam Days on Bioanalysis" provide an excellent platform for researchers, clinicians, and entrepreneurs to discuss diagnostics' hot topics, trends, and recent developments as well as new areas of application and future markets.
Often, the implementation of goal-oriented and profitable cooperations is quite a challenge, in particular when it comes to innovative product developments in the fields of diagnostics and analytics. Typically, challenges lie within finding the right cooperation partners, identifying…
More Releases for GDP
IATA's CEIV-Pharma Certification or HSA's GDP Certification?
Players throughout the pharmaceutical supply chain are experiencing growing pressure to gain accreditation for handling the high-value, temperature-sensitive cargo, as having a certification becomes more commonplace, rather than an exceptional feat in the industry.
There are currently 2 different measures of standards, one is IATA's CEIV-Pharma Certification and the other is HSA's GDP Certification.
There are major players on both sides of the fence which hold – GDP-compliant certificates and CEIV-pharma certifications.…
Exploring the recent PQG / ECA guide to GDP
The Pharmaceutical Quality Group (PQG) issued a monograph on Pharmaceutical Distribution in 1997 and initiated planning to revise this in line with the new EU GDP regulatory guideline (a final revised version was issued in March 2013 with an effective date of 8 September 2013).
Although the 2013 EU-GDP guidelines provide far more details than previous guidelines, it is clear that there was and is a need for further guidance on…
Tire Cord and Tire Fabrics Market - Sluggish GDP growth over 2025
Global Tire Cord and Tire Fabrics Market: Snapshot
Tire cords are the fundamental tire fortification materials, which helps in keeping up tire's dimension under various physical conditions and accordingly brings about comfortable riding. For instance, nylon tire cord, which are set up from high tenacity incessant filament yarn by means of handling and curving, gives high quality, weariness resistance, affect resistance, and high attachment. As the automotive in flourishes with rising…
Is demonetization responsible for lower GDP growth rate?
Decimal Point Analytics has released an weekly digest on 4th September titled ‘Is demonetization responsible for lower GDP growth rate?’
In this piece, Decimal point has weighed upon the recent announcement of GDP numbers which has been below market expectation.
Its arguments are counter to the popular consensus, that demonetization has been the prime reason for this considerable fall in GDP. The author has highlighted the fact that it’s the businesses…
European GDP Association nominates new Advisory Board Member
So far the Board of the European GDP Association comprised four members from the industry side, supported by a representative from the Finnish Medicines Agency FIMEA. Now a new Board Member has been nominated on the industry side.
Dr Laura Ribeiro, who is a Responsible Person at ID Logistics (formerly Logiters) in Portugal, has recently accepted her nomination as the fifth member of the GDP Association's Board. Prior to her…
US GDP Up 1.7% Shattering Economists Predictions
Aug. 01, 2013 - HONG KONG -- On Wednesday the US Commerce Department released its highly anticipated report on GDP, stating that in the second quarter the US economy grew 1.7% driven by an increase of 1.8% in consumer spending and 9% in business investment. New home construction was another important driver of growth, with investment rising 13.4%, the fourth consecutive increase. This gain beat expectations of a 1%…
